Protective effects of natural and partially degraded konjac glucomannan on Bifidobacteria against antibiotic damage

Carbohydr Polym. 2018 Feb 1;181:368-375. doi: 10.1016/j.carbpol.2017.10.083. Epub 2017 Oct 27.


This study was to evaluate the protective effects of a dietary fiber, konjac glucomannan (KGM) from the plant tuber of Amorphohallus konjac on Bifidobacteria against antibiotic damage. KGM (∼8.8×108Da) was partially degraded with high-intensity ultrasound to KGM-US (∼1.8×106Da) and then hydrolyzed with trifluoroacetic acid (TFA) to KGM-AH (1369Da). KGM-US (at 5g/l) showed the most significant protective effect on most bifidobacterial strains against penicillin and streptomycin inhibition, increasing the minimal inhibitory and bactericidal concentration (MIC and MBC) dramatically, and KGM also showed significant effects on enhancing the MBC of enrofloxacin, penicillin, tetracycline and streptomycin. In addition, the adsorbance ability and biofilm formation effects of KGM and degraded KGM products may be partially responsible for the protective effects. The results suggested that natural KGM and ultrasound treated KGM have protective effects for the human gut probiotic bacteria against the damage caused by specific antibiotics.

Keywords: Antibiotics; Bifidobacteria; Konjac glucomannan; Partial degradation; Prebiotic fiber.

MeSH terms

  • Adsorption
  • Amorphophallus / chemistry*
  • Anti-Bacterial Agents / pharmacology*
  • Bacterial Adhesion / drug effects
  • Bifidobacterium / drug effects*
  • Bifidobacterium / growth & development
  • Biofilms / drug effects
  • Inulin
  • Mannans / pharmacology*
  • Microbial Sensitivity Tests
  • Molecular Weight
  • Protective Agents / pharmacology*
  • Trisaccharides / chemistry
  • Ultrasonics
  • Viscosity


  • Anti-Bacterial Agents
  • Mannans
  • Protective Agents
  • Trisaccharides
  • (1-6)-alpha-glucomannan
  • Inulin