Chemopreventive Potential of Raw and Roasted Pistachios Regarding Colon Carcinogenesis

Nutrients. 2017 Dec 18;9(12):1368. doi: 10.3390/nu9121368.

Abstract

Pistachios are rich in health-promoting bioactive compounds such as B vitamins, γ-tocopherol, polyphenols and dietary fiber, which could contribute to the reduction of colon cancer risk in terms of chemoprevention (Fischer, S.; Glei, M. Health-Potential of Nuts. Ernaehrungs Umsch. Int. 2013, 60, 206-215.). Since pistachios are often consumed roasted, the present study aims at investigating the influence of different roasting conditions (RC) on potential chemopreventive effects of pistachios in colon adenoma cells such as growth and apoptosis, genotoxic- and anti-genotoxic effects and modulation of gene expression of detoxifying enzymes (CAT, SOD2, GPx1, and GSTP1). Fermentation supernatants (FS) were obtained from raw and roasted (RC1 = 141 °C/25 min, RC2 = 160 °C/15 min and RC3 = 185 °C/21 min) pistachios after in vitro fermentation. FS of pistachios significantly reduced LT97 cell growth in a time- and dose-dependent manner. Compared to the blank control, pistachio FS (2.5%) led to a significant average reduction of H₂O₂-induced DNA damage (1.5-fold). Levels of CAT mRNA were significantly increased (1.3-fold, on average for 5% FS). Pistachio FS (5%) significantly increased the number of early apoptotic cells (up to 2.1-fold) and levels of caspase-3 activities (up to 6.9-fold). The present results confirm a chemopreventive potential of pistachios, which is mediated by growth inhibition, induction of apoptosis and anti-genotoxic effects, as well as induction of CAT. These effects remain mostly unaffected by roasting.

Keywords: apoptosis; colon cancer; dietary fiber; pistachio.

MeSH terms

  • Anticarcinogenic Agents / analysis
  • Antioxidants / analysis
  • Apoptosis
  • Catalase / metabolism
  • Cell Line, Tumor
  • Cell Proliferation
  • Colon / cytology
  • Colon / metabolism
  • Colonic Neoplasms / prevention & control*
  • Comet Assay
  • DNA Damage
  • Dietary Fiber / analysis
  • Fermentation
  • Food Handling
  • Glutathione Peroxidase / metabolism
  • Glutathione S-Transferase pi / metabolism
  • Humans
  • Lutein / analysis
  • Nuts / chemistry*
  • Pistacia / chemistry*
  • Polyphenols / analysis
  • Superoxide Dismutase / metabolism
  • Vitamin B Complex / analysis
  • Zeaxanthins / analysis
  • gamma-Tocopherol / analysis

Substances

  • Anticarcinogenic Agents
  • Antioxidants
  • Dietary Fiber
  • Polyphenols
  • Zeaxanthins
  • Vitamin B Complex
  • gamma-Tocopherol
  • glutathione peroxidase GPX1
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • superoxide dismutase 2
  • GSTP1 protein, human
  • Glutathione S-Transferase pi
  • Lutein