A hydrophobic anchor mechanism defines a deacetylase family that suppresses host response against YopJ effectors

Nat Commun. 2017 Dec 19;8(1):2201. doi: 10.1038/s41467-017-02347-w.

Abstract

Several Pseudomonas and Xanthomonas species are plant pathogens that infect the model organism Arabidopsis thaliana and important crops such as Brassica. Resistant plants contain the infection by rapid cell death of the infected area through the hypersensitive response (HR). A family of highly related α/β hydrolases is involved in diverse processes in all domains of life. Functional details of their catalytic machinery, however, remained unclear. We report the crystal structures of α/β hydrolases representing two different clades of the family, including the protein SOBER1, which suppresses AvrBsT-incited HR in Arabidopsis. Our results reveal a unique hydrophobic anchor mechanism that defines a previously unknown family of protein deacetylases. Furthermore, this study identifies a lid-loop as general feature for substrate turnover in acyl-protein thioesterases and the described family of deacetylases. Furthermore, we found that SOBER1's biological function is not restricted to Arabidopsis thaliana and not limited to suppress HR induced by AvrBsT.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Acetylation
  • Arabidopsis / enzymology*
  • Arabidopsis / genetics
  • Arabidopsis / immunology
  • Arabidopsis / microbiology
  • Arabidopsis Proteins / chemistry*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / immunology
  • Carboxylic Ester Hydrolases / chemistry*
  • Carboxylic Ester Hydrolases / genetics
  • Carboxylic Ester Hydrolases / immunology
  • Crystallography, X-Ray
  • Host-Pathogen Interactions / physiology*
  • Hydrophobic and Hydrophilic Interactions
  • Molecular Docking Simulation
  • Mutation
  • Phylogeny
  • Plant Diseases / immunology*
  • Plant Diseases / microbiology
  • Plants, Genetically Modified
  • Protein Structure, Tertiary
  • Pseudomonas syringae / pathogenicity
  • Pseudomonas syringae / physiology
  • Substrate Specificity
  • Tobacco / genetics
  • Tobacco / microbiology
  • Transcription Activator-Like Effectors / immunology*
  • Transcription Activator-Like Effectors / metabolism
  • Xanthomonas / pathogenicity
  • Xanthomonas / physiology

Substances

  • Arabidopsis Proteins
  • Transcription Activator-Like Effectors
  • Carboxylic Ester Hydrolases
  • SOBER1 protein, Arabidopsis