Abstract
In recent years, avian influenza virus H9N2 undergoing antigenic drift represents a threat to poultry farming as well as public health. Current vaccines are restricted to inactivated vaccine strains and their related variants. In this study, a recombinant H9N2 (H9N2-TM) strain with a replaced H3 hemagglutinin (HA) transmembrane (TM) domain was generated. Virus assembly and viral protein composition were not affected by the transmembrane domain replacement. Further, the recombinant TM-replaced H9N2-TM virus could provide better inter-clade protection in both mice and chickens against H9N2, suggesting that the H3-TM-replacement could be considered as a strategy to develop efficient subtype-specific H9N2 influenza vaccines.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Viral / blood
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Antibodies, Viral / immunology
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Chickens
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Cross Protection*
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Female
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Hemagglutinin Glycoproteins, Influenza Virus / genetics
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Hemagglutinin Glycoproteins, Influenza Virus / immunology*
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Hemagglutinin Glycoproteins, Influenza Virus / metabolism
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Influenza A Virus, H9N2 Subtype / immunology*
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Influenza Vaccines / administration & dosage
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Influenza Vaccines / immunology*
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Mice
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Mice, Inbred BALB C
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Orthomyxoviridae Infections / prevention & control*
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Orthomyxoviridae Infections / virology
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Protein Domains
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology*
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Recombinant Proteins / metabolism
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Virus Replication
Substances
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Antibodies, Viral
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Hemagglutinin Glycoproteins, Influenza Virus
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Influenza Vaccines
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Recombinant Proteins