Cloning, Expression, and Purification of the Glycosylated Transmembrane Protein, Cation-Dependent Mannose 6-Phosphate Receptor, from Sf9 Cells Using the Baculovirus System

Methods Mol Biol. 2018;1722:105-116. doi: 10.1007/978-1-4939-7553-2_7.

Abstract

The cation-dependent mannose 6-phosphate receptor (CD-MPR) is a single-pass type I membrane protein. This protein functions to transport lysosomal enzymes displaying phosphomannosyl residues from the Golgi complex and the cell surface to the lysosome. This glycosylated protein contains three disulfide bridges in its 159-residue extracytoplasmic domain. One of the problems with studying eukaryotic membrane proteins is isolating sufficient quantities. Structural studies typically require several hundred milligrams of highly purified protein. Here we present a method to isolate milligram quantities of CD-MPR/Asn81 suitable for structural studies.

Keywords: CD-MPR; Glycoprotein; Purification; Sf9 cells; Transmembrane protein.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Baculoviridae
  • Cell Membrane / chemistry
  • Cloning, Molecular
  • Genetic Vectors
  • Glycosylation
  • Lysosomes / metabolism
  • Protein Transport
  • Receptor, IGF Type 2 / biosynthesis*
  • Receptor, IGF Type 2 / chemistry
  • Receptor, IGF Type 2 / genetics
  • Receptor, IGF Type 2 / isolation & purification*
  • Recombinant Proteins / biosynthesis*
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / isolation & purification*
  • Sf9 Cells

Substances

  • Receptor, IGF Type 2
  • Recombinant Proteins
  • cation-dependent mannose-6-phosphate receptor