Extracellular Matrix Molecule-Based Capture of Mesenchymal Stromal Cells Under Flow

Methods Mol Biol. 2018;1722:249-260. doi: 10.1007/978-1-4939-7553-2_16.

Abstract

We present a method to capture mesenchymal stromal cells (MSCs) by adhesion to extracellular matrix (ECM) molecules under flow conditions. The technique simulates a physiological system and exploits the natural biological interactions of cells, through integrin receptors, with their ECM. The system offers an insight into how MSCs could be targeted/localized to the site of interest (graft) following intravenous injection.

Keywords: Adhesion; Fibronectin; Laminin; Mesenchymal stromal cell.

MeSH terms

  • Cell Adhesion / physiology
  • Cell Differentiation / physiology
  • Cells, Immobilized / metabolism
  • Extracellular Matrix / physiology*
  • Fibronectins / metabolism
  • Flow Cytometry / methods*
  • Fluorescent Antibody Technique
  • Humans
  • Image Processing, Computer-Assisted
  • Integrins / metabolism
  • Laminin / metabolism
  • Mesenchymal Stem Cells / classification
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / metabolism*
  • Microscopy, Video
  • RNA
  • Single-Cell Analysis

Substances

  • Fibronectins
  • Integrins
  • Laminin
  • RNA