Ecological Balance of Oral Microbiota Is Required to Maintain Oral Mesenchymal Stem Cell Homeostasis

Stem Cells. 2018 Apr;36(4):551-561. doi: 10.1002/stem.2762. Epub 2018 Jan 4.

Abstract

Oral microbiome is essential for maintenance of oral cavity health. Imbalanced oral microbiome causes periodontal and other diseases. It is unknown whether oral microbiome affect oral stem cells function. This study used a common clinical antibiotic treatment approach to alter oral microbiome ecology and examine whether oral mesenchymal stem cells (MSCs) are affected. We found that altered oral microbiome resulted gingival MSCs deficiency, leading to a delayed wound healing in male mice. Mechanistically, oral microbiome release lipopolysaccharide (LPS) that stimulates the expression of microRNA-21 (miR-21) and then impair the normal function of gingival MSCs and wound healing process through miR-21/Sp1/telomerase reverse transcriptase pathway. This is the first study indicate that interplay between oral microbiome and MSCs homeostasis in male mice. Stem Cells 2018;36:551-561.

Keywords: Mesenchymal stem cells; Oral microbiota; Proliferation; Wound healing.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Lipopolysaccharides / metabolism*
  • Male
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / metabolism*
  • Mice
  • MicroRNAs / metabolism*
  • Microbiota*
  • Mouth / microbiology*
  • Sp1 Transcription Factor / metabolism*
  • Telomerase / metabolism*

Substances

  • Lipopolysaccharides
  • MIRN21 microRNA, mouse
  • MicroRNAs
  • Sp1 Transcription Factor
  • Telomerase
  • Tert protein, mouse