Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia

Rev Inst Med Trop Sao Paulo. 2017 Dec 21;59:e76. doi: 10.1590/S1678-9946201759076.


A Lateral Flow Assay to detect cryptococcal antigen (CrAg® LFA) in serum and cerebrospinal fluid for the rapid diagnosis of cryptococcosis was evaluated. A retrospective validation was performed. Sensitivity and specificity of the CrAg® LFA was 100%. High concordance (kappa index=1.0) between Cryptococcal Antigen Latex Agglutination System (CALAS®) and CrAg® LFA was observed. CrAg® LFA showed higher analytical sensitivity for detecting low concentrations of cryptococcal antigen.

Publication types

  • Evaluation Study

MeSH terms

  • Antigens, Fungal / blood*
  • Antigens, Fungal / cerebrospinal fluid*
  • Chromatography, Affinity / methods*
  • Colombia
  • Cryptococcosis / blood
  • Cryptococcosis / cerebrospinal fluid
  • Cryptococcosis / diagnosis*
  • Cryptococcus / immunology*
  • Humans
  • Point-of-Care Testing
  • Reproducibility of Results
  • Retrospective Studies
  • Sensitivity and Specificity


  • Antigens, Fungal