Lymphoblast-derived integration-free ISRM-CON9 iPS cell line from a 75year old female

Stem Cell Res. 2018 Jan;26:76-79. doi: 10.1016/j.scr.2017.12.007. Epub 2017 Dec 13.

Abstract

Human lymphoblast cells were used to generate integration-free induced pluripotent stem cells (iPSCs) employing episomal-based plasmids expressing OCT4, SOX2, NANOG, LIN28, c-MYC and L-MYC. The derived iPSCs were defined as pluripotent based on (i) expression of pluripotency-associated markers, (ii) embryoid body-based differentiation into cell types representative of the three germ layers and (iii) the similarity between the transcriptomes of the iPSC line and the human embryonic stem cell line H1 with a Pearson correlation of 0.95.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Biomarkers / metabolism
  • Cell Differentiation*
  • Cell Line
  • Cellular Reprogramming*
  • Embryoid Bodies / cytology*
  • Embryoid Bodies / metabolism
  • Female
  • Hematopoietic Stem Cells / cytology*
  • Hematopoietic Stem Cells / metabolism
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / metabolism
  • Karyotyping
  • Lymphocytes / cytology*
  • Lymphocytes / metabolism

Substances

  • Biomarkers