The role of bone marrow mononuclear cell-conditioned medium in the proliferation and migration of human dermal fibroblasts

Cell Mol Biol Lett. 2017 Dec 19;22:29. doi: 10.1186/s11658-017-0055-z. eCollection 2017.


Background: Several recent studies have demonstrated the great potential of bone marrow cells in regenerative medicine, not only for their ability to differentiate to match a damaged cell type, but also because they synthesize and release various growth factors and cytokines.We examined the effect of bone marrow cell-conditioned medium in the healing process, especially in terms of fibroblast proliferation and migration.

Methods: These in vitro studies consisted of co-culture (without direct contact) of dermal fibroblasts with mononuclear bone marrow cells and the use of conditioned medium obtained from these cultures in a scratch wound model.

Results: Mononuclear cells were found to increase the proliferation of fibroblasts, and the conditioned medium showed a stimulatory effect on the migration of fibroblasts.

Conclusion: When considered together with the observed increase in growth factor levels in conditioned medium, it appears that these cells act through a paracrine mechanism.

Keywords: Bone marrow mononuclear cells; Cell migration and proliferation; Human dermal fibroblasts; Paracrine interactions; Wound repair.

MeSH terms

  • Adult
  • Bone Marrow Cells / cytology*
  • Cell Movement / drug effects*
  • Cell Proliferation / drug effects
  • Culture Media, Conditioned / pharmacology*
  • Dermis / cytology*
  • Fibroblasts / cytology*
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Humans
  • Leukocytes, Mononuclear / cytology*
  • Leukocytes, Mononuclear / drug effects
  • Leukocytes, Mononuclear / metabolism
  • Solubility
  • Wound Healing / drug effects


  • Culture Media, Conditioned