Low-input Capture-C: A Chromosome Conformation Capture Assay to Analyze Chromatin Architecture in Small Numbers of Cells

Bio Protoc. 2017 Dec 5;7(23):e2645. doi: 10.21769/BioProtoc.2645.


Chromosome conformation capture (3C) techniques are crucial to understanding tissue-specific regulation of gene expression, but current methods generally require large numbers of cells. This protocol describes two new low-input Capture-C approaches that can generate high-quality 3C interaction profiles from 10,000-20,000 cells, depending on the resolution used for analysis.

Keywords: Capture-C; Chromatin architecture; Chromosome conformation capture (3C); Low input; Nuclear organization; Small cell numbers.