ExoCET: exonuclease in vitro assembly combined with RecET recombination for highly efficient direct DNA cloning from complex genomes

Nucleic Acids Res. 2018 Mar 16;46(5):2697. doi: 10.1093/nar/gkx1296.

Hailong Wang¹, Zhen Li¹, Ruonan Jia¹, Jia Yin¹, Aiying Li¹, Liqiu Xia², Yulong Yin^{1

2

3}, Rolf Müller^{1

4}, Jun Fu¹, A Francis Stewart⁵, Youming Zhang¹

¹ Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Shanda Nanlu 27, 250100 Jinan, People's Republic of China.
² State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, 410081 Changsha, People's Republic of China.
³ Key Laboratory of Subtropical Agro-ecology, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan Province 410125, People's Republic of China.
⁴ Department of Microbial Natural Products, Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research (HZI) and Pharmaceutical Biotechnology, Saarland University, Campus C2 3, 66123 Saarbrücken, Germany.
⁵ Genomics, Biotechnology Center, Center for Molecular and Cellular Bioengineering, Technische Universität Dresden, Tatzberg 47-51, 01307 Dresden, Germany.

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