Measuring Oxidative Stress in Caenorhabditis elegans: Paraquat and Juglone Sensitivity Assays

Bio Protoc. 2017 Jan 5;7(1):e2086. doi: 10.21769/BioProtoc.2086.

Abstract

Oxidative stress has been proposed to be one of the main causes of aging and has been implicated in the pathogenesis of many diseases. Sensitivity to oxidative stress can be measured by quantifying survival following exposure to a reactive oxygen species (ROS)-generating compound such as paraquat or juglone. Sensitivity to oxidative stress is a balance between basal levels of ROS, the ability to detoxify ROS, and the ability to repair ROS-mediated damage.

Keywords: C. elegans; Oxidative stress; juglone; paraquat; reactive oxygen species; stress resistance.