Preparation of a broad-spectrum anti-zearalenone and its primary analogues antibody and its application in an indirect competitive enzyme-linked immunosorbent assay

Guoliang Dong; Yuanhu Pan; Yulian Wang; Saeed Ahmed; Zhenli Liu; Dapeng Peng; Zonghui Yuan

doi:10.1016/j.foodchem.2017.12.016

Preparation of a broad-spectrum anti-zearalenone and its primary analogues antibody and its application in an indirect competitive enzyme-linked immunosorbent assay

Food Chem. 2018 May 1:247:8-15. doi: 10.1016/j.foodchem.2017.12.016. Epub 2017 Dec 7.

Authors

Guoliang Dong¹, Yuanhu Pan¹, Yulian Wang¹, Saeed Ahmed¹, Zhenli Liu¹, Dapeng Peng², Zonghui Yuan¹

Affiliations

¹ National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for Detection of Veterinary Drug Residues, MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
² National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for Detection of Veterinary Drug Residues, MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Huazhong Agricultural University, Wuhan, Hubei 430070, China. Electronic address: pengdapeng@mail.hzau.edu.cn.

PMID: 29277231
DOI: 10.1016/j.foodchem.2017.12.016

Abstract

A broad-spectrum monoclonal antibody (mAb)-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) has been developed for rapidly screening zearalenone (ZEN) and its primary analogues in various samples using an easy sample preparation procedure. Primarily, a group-specific mAb, 6C2, was produced, which had IC₅₀ values for ZEN, α-zearalenol, β-zearalenol, α-zearalanol, β-zearalanol and zearalanone of 114.0, 127.4, 290.4, 114.9, 205.6 and 257.1 ng L^-1, respectively. The limit of detection and limit of quantitation of this method for ZEN and its five primary analogues in various matrix samples ranged from 114.2 to 812.3 ng L^-1 and 237.1 to 1653.9 ng L^-1, respectively. The recoveries of the above samples spiked with ZEN and its five primary analogues were in the range of 62.9-113.6%. The CVs were less than 13.2%. A good correlation (R² = 0.995) between the ic-ELISA results and the HPLC-MS/MS results for swine feeds supported the reliability of the developed ic-ELISA.

Keywords: Animal edible tissue; Feed; Indirect competitive enzyme-linked immunosorbent assay; Monoclonal antibody; Zearalenone.

MeSH terms

Animal Feed / analysis
Animals
Antibodies, Monoclonal / immunology*
Chromatography, High Pressure Liquid / methods
Enzyme-Linked Immunosorbent Assay / methods*
Female
Food Contamination / analysis
Haptens / chemistry
Haptens / immunology
Inhibitory Concentration 50
Mice, Inbred BALB C
Reproducibility of Results
Sensitivity and Specificity
Swine
Tandem Mass Spectrometry / methods
Zea mays / chemistry
Zearalenone / analysis*
Zearalenone / immunology*
Zeranol / analogs & derivatives
Zeranol / immunology

Substances

Antibodies, Monoclonal
Haptens
zearalenol
Zearalenone
Zeranol
taleranol