Abstract
Recently, several innovative approaches have been developed that allow one to directly screen or select for improved protein folding in the cellular context. These methods have the potential of not just leading to a better understanding of the in vivo folding process, they may also allow for improved production of proteins of biotechnological interest.
Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Biosensing Techniques / methods*
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Biotechnology / methods
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Chaperonin 10 / chemistry*
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Chaperonin 10 / genetics
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Chaperonin 10 / metabolism
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Chaperonin 60 / chemistry*
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Chaperonin 60 / genetics
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Chaperonin 60 / metabolism
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Directed Molecular Evolution / methods*
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Genes, Reporter
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Kinetics
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Protein Engineering / methods*
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Protein Folding
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Protein Stability
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Staining and Labeling / methods*
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Thermodynamics
Substances
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Chaperonin 10
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Chaperonin 60
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Green Fluorescent Proteins