Regulation of posterior body and epidermal morphogenesis in zebrafish by localized Yap1 and Wwtr1

Elife. 2017 Dec 28;6:e31065. doi: 10.7554/eLife.31065.

Abstract

The vertebrate embryo undergoes a series of dramatic morphological changes as the body extends to form the complete anterior-posterior axis during the somite-forming stages. The molecular mechanisms regulating these complex processes are still largely unknown. We show that the Hippo pathway transcriptional coactivators Yap1 and Wwtr1 are specifically localized to the presumptive epidermis and notochord, and play a critical and unexpected role in posterior body extension by regulating Fibronectin assembly underneath the presumptive epidermis and surrounding the notochord. We further find that Yap1 and Wwtr1, also via Fibronectin, have an essential role in the epidermal morphogenesis necessary to form the initial dorsal and ventral fins, a process previously thought to involve bending of an epithelial sheet, but which we now show involves concerted active cell movement. Our results reveal how the Hippo pathway transcriptional program, localized to two specific tissues, acts to control essential morphological events in the vertebrate embryo.

Keywords: Fibronectin; Hippo pathway; developmental biology; fin formation; morphogenesis; stem cells; vertebrate embryo elongation; zebrafish.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Epidermis / embryology*
  • Fibronectins / metabolism
  • Gene Expression Regulation, Developmental*
  • Intracellular Signaling Peptides and Proteins / metabolism*
  • Morphogenesis*
  • Notochord / embryology*
  • Trans-Activators / metabolism*
  • Zebrafish / embryology*
  • Zebrafish Proteins / metabolism*

Substances

  • Fibronectins
  • Intracellular Signaling Peptides and Proteins
  • Trans-Activators
  • WWTR1 protein, zebrafish
  • Yes-associated protein (yap), zebrafish
  • Zebrafish Proteins