Reaction of Toxic Shock Syndrome Toxin 1 With Endothelium of Human Umbilical Cord Vein

Rev Infect Dis. Jan-Feb 1989;11 Suppl 1:S282-7; discussion S287-8. doi: 10.1093/clinids/11.supplement_1.s282.

Abstract

The binding of toxic shock syndrome toxin 1 (TSST-1) to the endothelium of human umbilical cord veins and arteries was studied. Radiolabeled TSST-1, perfused through a segment of human umbilical cord vein, bound to the vessel at 4 degrees C, with a dissociation constant (Kd) of 6.5 X 10(-10) M. A pre-embedding electron-microscopic immunogold technique revealed that TSST-1 was located in the veins and arteries on the surface of endothelial cells and in the area of cell contacts. Umbilical cord vein segments filled with TSST-1 were incubated for 30 minutes at 37 degrees C, fixed by perfusion and embedded in hydrophilic resin (Lowicryl K4M). Postembedding immunogold staining of ultrathin sections showed TSST-1 in the vesicles crossing the cytoplasm of the veins' endothelial cells and entering underlying elastic and collagen layers.

MeSH terms

  • Bacterial Toxins*
  • Electrophoresis, Polyacrylamide Gel
  • Endothelium, Vascular / metabolism*
  • Endothelium, Vascular / ultrastructure
  • Enterotoxins / metabolism*
  • Humans
  • Immunohistochemistry
  • Microscopy, Electron
  • Superantigens*
  • Umbilical Veins / metabolism
  • Umbilical Veins / ultrastructure

Substances

  • Bacterial Toxins
  • Enterotoxins
  • Superantigens
  • enterotoxin F, Staphylococcal