MiR-96 regulates bone metabolism by targeting osterix

Clin Exp Pharmacol Physiol. 2018 Jun;45(6):602-613. doi: 10.1111/1440-1681.12912. Epub 2018 Feb 14.


MicroRNAs (miRNAs) play important roles in bone metabolism and aging. Here we show that miR-96 was markedly up-regulated in serum of elderly patients with osteoporosis by miRNA microarray analysis and qRT-PCR. Moreover miR-96 was also up-regulated in bone marrow mesenchymal stem cells (BMSCs) of aged humans and mice. Our results show that the over-expression of miR-96 reduced osteogenic differentiation of BMSCs, whereas the inhibition of miR-96 increased osteogenic differentiation of BMSCs. At the molecular level, miR-96 regulated osteogenesis by targeting osterix. Interestingly, over-expression of miR-96 in young mice by intravenous injection of agomiR-96 developed a low bone mass due to impaired osteogenesis. However, inhibition of miR-96 in aged mice attenuated the age-related bone loss. Thus, our data suggest that miR-96 regulates osteogenesis and may represent a potential diagnostic marker or therapeutic target for age-related bone loss.

Keywords: bone; metabolism; microRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aging / genetics
  • Aging / metabolism
  • Animals
  • Base Sequence
  • Bone and Bones / metabolism*
  • Female
  • Humans
  • Male
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / metabolism
  • Mice
  • MicroRNAs / genetics*
  • Osteogenesis / genetics
  • Osteoporosis / genetics
  • Osteoporosis / metabolism
  • Osteoporosis / pathology
  • Osteoporosis / physiopathology
  • Sp7 Transcription Factor / genetics*
  • Up-Regulation
  • Young Adult


  • MIRN96 microRNA, human
  • MicroRNAs
  • Mirn96 microRNA, mouse
  • Sp7 Transcription Factor

Associated data

  • GENBANK/NM_001146038
  • GENBANK/NM_130458.4
  • GENBANK/NM_007393.5