An OPR3-independent pathway uses 4,5-didehydrojasmonate for jasmonate synthesis

Nat Chem Biol. 2018 Feb;14(2):171-178. doi: 10.1038/nchembio.2540. Epub 2018 Jan 1.


Biosynthesis of the phytohormone jasmonoyl-isoleucine (JA-Ile) requires reduction of the JA precursor 12-oxo-phytodienoic acid (OPDA) by OPDA reductase 3 (OPR3). Previous analyses of the opr3-1 Arabidopsis mutant suggested an OPDA signaling role independent of JA-Ile and its receptor COI1; however, this hypothesis has been challenged because opr3-1 is a conditional allele not completely impaired in JA-Ile biosynthesis. To clarify the role of OPR3 and OPDA in JA-independent defenses, we isolated and characterized a loss-of-function opr3-3 allele. Strikingly, opr3-3 plants remained resistant to necrotrophic pathogens and insect feeding, and activated COI1-dependent JA-mediated gene expression. Analysis of OPDA derivatives identified 4,5-didehydro-JA in wounded wild-type and opr3-3 plants. OPR2 was found to reduce 4,5-didehydro-JA to JA, explaining the accumulation of JA-Ile and activation of JA-Ile-responses in opr3-3 mutants. Our results demonstrate that in the absence of OPR3, OPDA enters the β-oxidation pathway to produce 4,5-ddh-JA as a direct precursor of JA and JA-Ile, thus identifying an OPR3-independent pathway for JA biosynthesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Alternaria
  • Animals
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / metabolism
  • Biological Assay
  • Cyclopentanes / metabolism*
  • Disease Resistance*
  • Gene Expression Profiling
  • Gene Expression Regulation, Plant
  • Homozygote
  • Insecta
  • Isoleucine / analogs & derivatives*
  • Isoleucine / metabolism
  • Mutation
  • Oxylipins / metabolism*
  • Plant Diseases / prevention & control*
  • Plant Growth Regulators / metabolism
  • Plant Roots / metabolism
  • Signal Transduction


  • Arabidopsis Proteins
  • Cyclopentanes
  • Oxylipins
  • Plant Growth Regulators
  • jasmonoyl-isoleucine
  • Isoleucine
  • jasmonic acid

Associated data

  • PubChem-Substance/348400933
  • PubChem-Substance/348400941
  • PubChem-Substance/348400942
  • PubChem-Substance/348400943
  • PubChem-Substance/348400944
  • PubChem-Substance/348400945
  • PubChem-Substance/348400946
  • PubChem-Substance/348400947
  • PubChem-Substance/348400948
  • PubChem-Substance/348400934
  • PubChem-Substance/348400935
  • PubChem-Substance/348400936
  • PubChem-Substance/348400937
  • PubChem-Substance/348400938
  • PubChem-Substance/348400939
  • PubChem-Substance/348400940