Cutaneous wound healing using polymeric surgical dressings based on chitosan, sodium hyaluronate and resveratrol. A preclinical experimental study

Colloids Surf B Biointerfaces. 2018 Mar 1:163:155-166. doi: 10.1016/j.colsurfb.2017.12.041. Epub 2017 Dec 21.


Wound healing is a complex and dynamic process that involves modifying the wound environment depending on the patient's health status. Scar formation depends on many factors that influence wound healing, are important to bear in mind because most of the negative factors involved can be stopped by implementing an adequate treatment. The consensus in wound therapy recommends dressings that should keep a moist and alkaline environment thus creating a protective barrier against mechanical stress and secondary infections, in view of promoting granulation. In the current paper, we aimed to synthesize a polymer-based sponge containing chitosan-sodium hyaluronate-resveratrol (CHR) and to evaluate its regenerative potential. The process of synthesizing the CHR polymer was described before microtomography analysis was conducted and the density and porosity of the obtained sponges was assessed. The cytotoxicity was evaluated in vitro. By undertaking the in vivo testing of the CHR polymer, we aimed to determine the CHR sponge's potential to stimulate tissue regeneration after inflicting a controlled, reproducible and measurable skin wound in an animal model. Skin punch biopsies were harvested from the healed area and were subjected to histopathological evaluation. The results obtained in this study confirmed that this polymer accelerates the formation of granulation facilitating wound healing, while also achieving a bacteriostatic outcome.

Keywords: Chitosan; Cytotoxicity; Granulation tissue; Polymer; Surgical dressing; Wound healing.

MeSH terms

  • Animals
  • Bandages*
  • Cell Line
  • Cell Survival / drug effects
  • Chitosan / chemistry*
  • Drug Evaluation, Preclinical
  • Fibroblasts / cytology
  • Fibroblasts / drug effects
  • Humans
  • Hyaluronic Acid / chemistry*
  • Male
  • Mice
  • Mice, Inbred ICR
  • Porosity
  • Resveratrol
  • Skin / drug effects*
  • Skin / injuries
  • Stilbenes / chemistry
  • Stilbenes / pharmacology*
  • Surgical Wound / drug therapy*
  • Surgical Wound / pathology
  • Wound Healing / drug effects*


  • Stilbenes
  • Hyaluronic Acid
  • Chitosan
  • Resveratrol