One-Step Partially Purified Lipases (ScLipA and ScLipB) From Schizophyllum Commune UTARA1 Obtained via Solid State Fermentation and Their Applications

Molecules. 2017 Dec 8;22(12):2106. doi: 10.3390/molecules22122106.

Abstract

Lipases with unique characteristics are of value in industrial applications, especially those targeting cost-effectiveness and less downstream processes. The aims of this research were to: (i) optimize the fermentation parameters via solid state fermentation (SSF); and (ii) study the performance in hydrolysis and esterification processes of the one-step partially purified Schizophyllum commune UTARA1 lipases. Lipase was produced by cultivating S. commune UTARA1 on sugarcane bagasse (SB) with used cooking oil (UCO) via SSF and its production was optimized using Design-Expert® 7.0.0. Fractions 30% (ScLipA) and 70% (ScLipB) which contained high lipase activity were obtained by stepwise (NH₄)₂SO₄ precipitation. Crude fish oil, coconut oil and butter were used to investigate the lipase hydrolysis capabilities by a free glycerol assay. Results showed that ScLipA has affinities for long, medium and short chain triglycerides, as all the oils investigated were degraded, whereas ScLipB has affinities for long chain triglycerides as it only degrades crude fish oil. During esterification, ScLipA was able to synthesize trilaurin and triacetin. Conversely, ScLipB was specific towards the formation of 2-mono-olein and triacetin. From the results obtained, it was determined that ScLipA and ScLipB are sn-2 regioselective lipases. Hence, the one-step partial purification strategy proved to be feasible for partial purification of S. commune UTARA1 lipases that has potential use in industrial applications.

Keywords: esterification; hydrolysis; sugarcane bagasse; used cooking oil.

MeSH terms

  • Esterification
  • Fermentation*
  • Fish Oils / chemistry
  • Glycerol / chemistry
  • Hydrolysis
  • Kinetics
  • Lipase / chemistry*
  • Lipase / isolation & purification
  • Lipase / metabolism
  • Oleic Acids / chemistry
  • Plant Oils / chemistry
  • Schizophyllum / enzymology*
  • Triacetin / chemistry
  • Triglycerides / chemistry

Substances

  • Fish Oils
  • Oleic Acids
  • Plant Oils
  • Triglycerides
  • trilaurin
  • Lipase
  • Glycerol
  • Triacetin