Establishment of a recombinant Escherichia coli-induced piglet diarrhea model

Front Biosci (Landmark Ed). 2018 Mar 1;23:1517-1534. doi: 10.2741/4658.


Enterotoxigenic Escherichia coli strains induce human and animal intestinal dysfunction and injury, and cause diarrhea in weanling pigs. A recombinant E.coli strain LMG194-STa which expressed single toxin STa of ETEC was constructed by directly inserting the STa gene of ETEC into the expression vector pBAD202 and then transferring the recombinant plasmid pBAD-STa into the E.coli LMG19 host strain. Diarrhea and intestinal injury in piglets were induced by oral administration of recombinant strain LMG194-Sta. In vitro, the recombinant strain LMG194-Sta had the same toxicity to the IPEC cell as wild type strain K88, and higher toxicity than the host strain LMG194. In vivo, LMG194-STa caused severe diarrhea in piglets as K88, the diarrhea rate of LMG194-STa and K88 groups was higher than that in the LMG194 and control groups. Both K88 and LMG194-STa induced intestinal inflammation, injury, and atrophy, while adversely affecting the expression of genes for cytokines, transporters and ion channels, and nutrient metabolism. Thus, we established a porcine model of recombinant E. coli strain LMG194-STa-induced diarrhea for future nutritional and mechanistic studies of intestinal dysfunction.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Bacterial Toxins / genetics
  • Bacterial Toxins / metabolism
  • DNA, Recombinant
  • Diarrhea / genetics
  • Diarrhea / microbiology
  • Diarrhea / veterinary*
  • Disease Models, Animal*
  • Enterotoxigenic Escherichia coli / genetics
  • Enterotoxigenic Escherichia coli / metabolism
  • Enterotoxigenic Escherichia coli / physiology*
  • Enterotoxins / genetics
  • Enterotoxins / metabolism
  • Escherichia coli Infections / genetics
  • Escherichia coli Infections / microbiology
  • Escherichia coli Infections / veterinary*
  • Escherichia coli Proteins / genetics
  • Escherichia coli Proteins / metabolism
  • Gene Expression Profiling
  • Host-Pathogen Interactions
  • Intestinal Mucosa / metabolism
  • Intestinal Mucosa / microbiology
  • Intestinal Mucosa / pathology
  • Swine
  • Swine Diseases / genetics
  • Swine Diseases / microbiology*


  • Bacterial Toxins
  • DNA, Recombinant
  • Enterotoxins
  • Escherichia coli Proteins
  • heat stable toxin (E coli)