Objective: To investigate the possible role of IL17-and IL22-secreting cells combined with patch test for the prediction of formaldehyde-induced occupational allergic contact dermatitis(OACD). Methods: From October 2014 to October 2016, totally 131 formaldehyde-exposed workers(49 cases with inflammatory skin lesions,82 ones without inflammatory skin lesions)and 63 non-exposed health controls were recruited. Patch-test was performed in 49 cases of formaldehyde-exposed workers with inflammatory skin lesions. Circulating IL17+and IL22+Tcell subsets were assessed by flow cytometry(FCM). Results: Among 49 cases of formaldehyde-exposed workers with inflammatory skin lesions,32 cases were with positive patch-test while 17 cases with negative patch-test. The proportions of circulating CD3+CD8-IL17+ and CD3+CD8-IL22+ cells from patch-test(+) formaldehyde-exposed workers were significantly higher than that of patch-test(-)group, formaldehyde-exposed workers without skin lesions and non-exposed controls(P<0.05). The proportions of circulating CD3+CD8-IL17+ and CD3+CD8-IL22+cells from patch-test(-)group and formaldehyde-exposed workers without skin lesions were also higher than that of non-exposed controls(P<0.05). But there was no significant difference between patch-test(-)group and formaldehyde-exposed workers without skin lesions(P>0.05). Peripheral CD3+CD8+IL17+and CD3+CD8+IL22+cells were also detected in spite of small amounts. The percentages of CD3+CD8+IL17+and CD3+CD8+IL22+ cells inperipheral blood from patch-test(+)formaldehyde-exposed workers were enhanced significantly, compared to patch-test(-)group, formaldehyde-exposed workers without skin lesions and non-exposed controls(P<0.05). The proportions of circulating CD3+CD8+IL17+ and CD3+CD8+IL22+ cells from patch-test(-)group and formaldehyde-exposed workers without skin lesions were significantly higher than that of non-exposed controls(P<0.05). But there was no significant difference between patch-test(-) group and formaldehyde-exposed workers without skin lesions(P>0.05). Conclusion: The proportions of circulating IL17+ and IL22+T cells(both CD8-and CD8+)are enhanced in formaldehyde-exposed workers at proposed OEL, possibly involved in the development of formaldehyde-induced OACD.The detection of IL17-and IL22-secreting cells combined with formaldehyde patch test help to screen the workers with allergy property and prevent OACD.
目的： 探讨外周血IL17、IL22分泌细胞检测，联合皮肤斑贴试验，对甲醛致职业性变应性接触性皮炎（OACD）发病的预测作用。 方法： 于2014年10月至2016年10月，选取131例职业性甲醛接触工人（有皮肤炎症改变的49例，无皮肤炎症改变的82例）作为接触组，63例健康工人作为对照组。对有皮肤炎症改变的甲醛接触工人行皮肤斑贴试验；采用流式细胞术（FCM）检测各组外周血IL17阳性（IL17+）及IL22阳性（IL22+）T细胞亚型并分析其所占比例。 结果： 49名有皮肤炎症改变的甲醛接触组工人中，斑贴试验阳性32例，阴性17例。斑贴试验阳性接触组外周血CD3+CD8-IL17+及CD3+CD8-IL22+细胞百分比高于斑贴试验阴性接触组、无皮肤改变接触组及对照组，斑贴试验阴性接触组及无皮肤改变接触组高于对照组，差异均有统计学意义（P<0.05），斑贴试验阴性接触组与无皮肤改变接触组差异无统计学意义（P>0.05）；外周血中尚存在少量CD3+CD8+IL17+及CD3+CD8+IL22+细胞，斑贴试验阳性接触组高于斑贴试验阴性接触组、无皮肤改变接触组及对照组，斑贴试验阴性接触组及无皮肤改变接触组高于对照组，差异有统计学意义（P<0.05），斑贴试验阴性接触组与无皮肤改变接触组差异无统计学意义（P>0.05）。 结论： 职业性甲醛接触人群外周血IL17+及IL22+T细胞增多，包括CD8-T细胞及CD8+T细胞，可能与OACD发病有关；外周血IL17及IL22分泌细胞检测联合斑贴试验可能有助于早期筛选出具有甲醛过敏体质的工人，预防OACD发生。.
Keywords: Dermatitis, allergic contact; Dermatitis, occupational; Formaldehyde; Interleukin17; Interleukin22.