1950MHz Radio Frequency Electromagnetic Radiation Inhibits Testosterone Secretion of Mouse Leydig Cells

Int J Environ Res Public Health. 2017 Dec 23;15(1):17. doi: 10.3390/ijerph15010017.


More studies that are focused on the bioeffects of radio-frequency (RF) electromagnetic radiation that is generated from the communication devices, but there were few reports with confirmed results about the bioeffects of RF radiation on reproductive cells. To explore the effects of 1950 MHz RF electromagnetic radiation (EMR) on mouse Leydig (TM3) cells. TM3 cells were irradiated or sham-irradiated continuously for 24 h by the specific absorption rate (SAR) 3 W/kg radiation. At 0, 1, 2, 3, 4, and 5 days after irradiation, cell proliferation was detected by cell counting kit-8 (CCK-8) method, cell cycle distribution, percentage of apoptosis, and cellular reactive oxygen species (ROS) were examined by flow cytometry, Testosterone level was measured using enzyme-linked immunosorbent assay (ELISA) assay, messenger ribonucleic acid (mRNA) expression level of steroidogenic acute regulatory protein (StAR) and P450scc in TM3 cells was detected by real-time polymerase chain reaction (PCR). After being irradiated for 24 h, cell proliferation obviously decreased and cell cycle distribution, secretion capacity of Testosterone, and P450scc mRNA level were reduced. While cell apoptosis, ROS, and StAR mRNA level did not change significantly. The current results indicated that 24 h of exposure at 1950 MHz 3 W/kg radiation could cause some adverse effects on TM3 cells proliferation and Testosterone secretion, further studies about the biological effects in the reproductive system that are induced by RF radiation are also needed.

Keywords: 1950 MHz; Leydig cell; radio frequency electromagnetic radiation; testosterone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / radiation effects
  • Cell Cycle / radiation effects
  • Cell Line
  • Cell Proliferation / radiation effects
  • Leydig Cells / metabolism
  • Leydig Cells / radiation effects*
  • Male
  • Mice
  • Phosphoproteins / genetics
  • Phosphoproteins / metabolism
  • RNA, Messenger / metabolism
  • Radio Waves / adverse effects*
  • Reactive Oxygen Species / metabolism
  • Testosterone / antagonists & inhibitors*
  • Testosterone / metabolism


  • Phosphoproteins
  • RNA, Messenger
  • Reactive Oxygen Species
  • steroidogenic acute regulatory protein
  • Testosterone