Antibody Screening Using High-Throughput Flow Cytometry

Cold Spring Harb Protoc. 2018 Jan 2;2018(1). doi: 10.1101/pdb.top093773.


Because molecular targets addressable with antibody therapeutics are present on the surface of cells or in circulation, they are ideal for screening by cell- or bead-based assays using flow cytometry, a powerful, high-content analysis technique for cells, beads, and other particles in suspension. The ability to analyze thousands of cells per second, combined with multiplexing capabilities, has made this technology indispensable for laboratories performing antibody development work. Advances in this field, particularly in the areas of plate-based sampling and high-throughput flow cytometry, are enabling the use of this technology earlier in the antibody development and discovery process.

MeSH terms

  • Animals
  • Antibodies / metabolism*
  • Antigens / analysis*
  • Flow Cytometry / methods*
  • High-Throughput Screening Assays*
  • Humans
  • Immunologic Factors / metabolism*
  • Protein Binding
  • Staining and Labeling / methods*


  • Antibodies
  • Antigens
  • Immunologic Factors