Objectives: The aim of this study was to assess the possibility of transmitting the "ampicillin" growth inhibitory property to pure sterile water.
Design: Three control groups were designed as references of bacterial maximal growth (MG). Different concentrations of ampicillin and pure sterile water used as "positive" and "negative" controls, respectively, and the bacterial cultures supplemented with water that merely exposed to the background carrier waves were set as "sham control."
Settings: The electronic characteristic of the ampicillin solution relayed to the water and then it was added to the bacterial culture medium. By this treatment, water received both the carrier wave and ampicillin electronic properties at 1-105 Hz range.
Results: When the MG of the "treated water" and "positive control" dilutions is getting normalized against either sham or negative control, the treated water competently imitates ampicillin in a way that there is no significant difference with the positive control. These results will be affected to some extent by the time of electronic transmission and the inoculation concentration of bacterial culture. Comparison of electromagnetic signal patterns of "treated water" with the "positive control" shows that they are more similar to each other rather than negative control.
Conclusion: It is believed that the extremely low frequencies can facilitate electronic transmission of ampicillin supramolecular chemistry into water. Imprinting such a property in water could be the result of durable structural configuration in treated water.
Keywords: antibacterial; extremely low frequency; supramolecular chemistry; water structure.