Abstract
UV light, especially UVB, is known as a trigger of allergic reaction, leading to mast cell degranulation and histamine release. In this study, phlorotannin Fucofuroeckol-A (F-A) derived from brown algal Ecklonia stolonifera Okamura was evaluated for its protective capability against UVB-induced allergic reaction in RBL-2H3 mast cells. It was revealed that F-A significantly suppress mast cell degranulation via decreasing histamine release as well as intracellular Ca2+ elevation at the concentration of 50 μM. Moreover, the inhibitory effect of F-A on IL-1β and TNF-α productions was also evidenced. Notably, the protective activity of F-A against mast cell degranulation was found due to scavenging ROS production. Accordingly, F-A from brown algal E. stolonifera was suggested to be promising candidate for its protective capability against UVB-induced allergic reaction.
Keywords:
Ecklonia stolonifera; Fucofuroeckol-A; anti-allergy; degranulation; mast cells; phlorotannin.
MeSH terms
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Animals
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Anti-Allergic Agents / chemistry
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Anti-Allergic Agents / isolation & purification
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Anti-Allergic Agents / pharmacology*
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Benzofurans / chemistry
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Benzofurans / isolation & purification
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Benzofurans / pharmacology*
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Calcium / metabolism
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Cell Degranulation / drug effects*
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Cell Degranulation / radiation effects
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Cell Line, Tumor
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Dioxins / chemistry
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Dioxins / isolation & purification
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Dioxins / pharmacology*
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Free Radical Scavengers / isolation & purification
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Free Radical Scavengers / pharmacology
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Histamine / metabolism
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Interleukin-1beta / antagonists & inhibitors
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Mast Cells / drug effects*
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Mast Cells / metabolism
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Mast Cells / radiation effects
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Phaeophyceae / metabolism*
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Rats
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Reactive Oxygen Species / metabolism
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Tumor Necrosis Factor-alpha / antagonists & inhibitors
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Ultraviolet Rays / adverse effects
Substances
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Anti-Allergic Agents
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Benzofurans
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Dioxins
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Free Radical Scavengers
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Interleukin-1beta
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Reactive Oxygen Species
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Tumor Necrosis Factor-alpha
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fucofuroeckol-A
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Histamine
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Calcium