CHROMIXS: automatic and interactive analysis of chromatography-coupled small-angle X-ray scattering data

Bioinformatics. 2018 Jun 1;34(11):1944-1946. doi: 10.1093/bioinformatics/btx846.


Summary: Size-exclusion chromatography (SEC) coupled to small-angle X-ray scattering (SAXS), also known as inline SEC-SAXS, is being increasingly used for the structural analysis of biological macromolecules, complexes and mixtures in solution. A single SEC-SAXS run generates thousands of individual SAXS profiles from the eluting solute and their analysis requires a correct identification of buffer and sample regions, a rather laborous task. We present CHROMIXS (as in CHROMatography Inline X-ray Scattering), a program for rapid reduction and analysis, both automatically and interactively, of SEC-SAXS data.

Availability and implementation: CHROMIXS is freely available to academic users as part of the ATSAS software suite (

Contact: or

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromatography / methods*
  • Protein Conformation
  • Scattering, Small Angle
  • Serum Albumin, Bovine / chemistry
  • Serum Albumin, Bovine / metabolism
  • Software*
  • X-Ray Diffraction / methods*


  • Serum Albumin, Bovine