Polymorphisms in oxidative pathway related genes and susceptibility to inflammatory bowel disease

World J Gastroenterol. 2017 Dec 21;23(47):8300-8307. doi: 10.3748/wjg.v23.i47.8300.

Abstract

Aim: To investigate whether common variants in the oxidative pathway genes influence inflammatory bowel disease (IBD) risk among Moroccan patients.

Methods: The distribution of (TAAA)n_rs12720460 and (CCTTT)n _rs3833912 NOS2A microsatellite repeats, HIF-1A_rs11549467 and NFKB1-94ins/delATTG_rs28362491 was analyzed in 507 subjects grouped in 199 IBD and 308 healthy controls. Genotyping was performed with polymerase chain reaction-fluorescent method and the TaqMan® allelic discrimination technology.

Results: The allele and genotype frequencies of HIF1A_ rs11549467, NFKB1_rs28362491 and NOS2A_ (TAAA)n did not differ significantly between patients and controls. Analysis of NOS2A_ (CCTTT)n markers evidenced differences between patients and healthy controls. A preferential presence of the (CCTTT)8 (P = 0.02; OR = 1.71, 95%CI: 1.07-2.74), (CCTTT)14 (P = 0.02; OR = 1.71, 95%CI: 1.06-2.76) alleles in IBD, (CCTTT)8 (P = 0.008; OR = 1.95, 95%CI: 1.17-3.23) in CD and (CCTTT)7 (P = 0.009; OR = 7.61, 95%CI: 1.25-46.08), (CCTTT)11 (P = 0.05; OR = 0.51, 95%CI: 0.25-1.01), (CCTTT)14 (P = 0.02; OR = 2.05, 95%CI: 1.07-3.94), (CCTTT)15 (P = 0.01; OR = 2.25, 95%CI: 1.16-4.35) repeats in UC patients indicated its possible association with higher disease risk which need to be confirmed in a larger sample size.

Conclusion: Our results suggest that the NOS2A_ (CCTTT)n gene variations may influence IBD susceptibility in the Moroccan population.

Keywords: HIF1A; Inflammatory bowel disease; Moroccan patients; NFKB1; NOS2A.

MeSH terms

  • Adolescent
  • Adult
  • Alleles
  • Female
  • Genetic Predisposition to Disease*
  • Haplotypes
  • Humans
  • Hypoxia-Inducible Factor 1, alpha Subunit / genetics*
  • Inflammatory Bowel Diseases / genetics*
  • Male
  • Microsatellite Repeats / genetics
  • Morocco
  • NF-kappa B p50 Subunit / genetics*
  • Nitric Oxide Synthase Type II / genetics*
  • Oxidative Stress / genetics*
  • Polymorphism, Single Nucleotide
  • Promoter Regions, Genetic
  • Young Adult

Substances

  • HIF1A protein, human
  • Hypoxia-Inducible Factor 1, alpha Subunit
  • NF-kappa B p50 Subunit
  • NFKB1 protein, human
  • NOS2 protein, human
  • Nitric Oxide Synthase Type II