Algorithm of Molecular and Biological Assessment of the Mechanisms of Sensitivity to Drug Toxicity by the Example of Cyclophosphamide

Bull Exp Biol Med. 2018 Jan;164(3):324-329. doi: 10.1007/s10517-018-3982-4. Epub 2018 Jan 8.

Abstract

Comparative study of the liver, blood, and spleen of DBA/2JSto and BALB/cJLacSto mice sensitive and resistant to acute toxicity of the cyclophosphamide allowed us to reveal basic toxicity biomarkers of this antitumor and immunosuppressive agent. Obtained results can be used for the development of an algorithm for evaluation of toxic effects of drugs and food components.

Keywords: BALB/cJLacSto and DBA/2JSto mice; acute toxicity; biomarkers; cyclophosphamide.

MeSH terms

  • Alcohol Dehydrogenase / genetics
  • Alcohol Dehydrogenase / metabolism
  • Aldehyde Dehydrogenase / genetics
  • Aldehyde Dehydrogenase / metabolism
  • Algorithms*
  • Animals
  • Cyclophosphamide / toxicity*
  • Cytochrome P-450 Enzyme System / genetics
  • Cytochrome P-450 Enzyme System / metabolism
  • Cytotoxins / toxicity*
  • Gene Expression Regulation / drug effects
  • Liver / drug effects*
  • Liver / metabolism
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred DBA
  • Microsomes, Liver / drug effects
  • Microsomes, Liver / metabolism
  • Organ Specificity
  • Species Specificity
  • Spleen / drug effects*
  • Spleen / metabolism
  • Toxicity Tests, Acute

Substances

  • Cytotoxins
  • Cyclophosphamide
  • Cytochrome P-450 Enzyme System
  • Alcohol Dehydrogenase
  • Aldehyde Dehydrogenase