New colony multiplex PCR assays for the detection and discrimination of vancomycin-resistant enterococcal species

J Microbiol Methods. 2018 Feb;145:69-72. doi: 10.1016/j.mimet.2017.12.013. Epub 2018 Jan 5.

Abstract

New colony multiplex PCR assays for detection of seven types of vancomycin-resistance determinants and eight types of Enterococcus species were developed. For 135 enterococcal isolates examined in this study, these assays showed high sensitivity and specificity, and could provide the rapid and accurate detection of vancomycin-resistant determinants and Enterococcus spp.

Keywords: Colony multiplex PCR; Drug resistance; Enterococcus spp.; Vancomycin-resistant enterococci.

MeSH terms

  • Bacterial Proteins / genetics
  • Enterococcus faecalis / drug effects
  • Enterococcus faecalis / isolation & purification*
  • Enterococcus faecium / drug effects
  • Enterococcus faecium / isolation & purification*
  • Enterococcus hirae / drug effects
  • Enterococcus hirae / isolation & purification*
  • Gram-Positive Bacterial Infections / diagnosis*
  • Gram-Positive Bacterial Infections / microbiology
  • Humans
  • Multiplex Polymerase Chain Reaction / methods
  • Peptide Synthases / genetics
  • Sensitivity and Specificity
  • Vancomycin / pharmacology
  • Vancomycin Resistance / drug effects
  • Vancomycin-Resistant Enterococci / drug effects
  • Vancomycin-Resistant Enterococci / isolation & purification*

Substances

  • Bacterial Proteins
  • Vancomycin
  • Peptide Synthases