Dermal Vγ4+T cells enhance the IMQ-induced psoriasis-like skin inflammatidon in re-challenged mice

Rong Zhu; Xiaowei Cai; Chunli Zhou; Yashu Li; Xiaorong Zhang; Yiqian Li; Lei Ran; Ke Huang; Weifeng He; Rupeng Wang

Dermal Vγ₄⁺T cells enhance the IMQ-induced psoriasis-like skin inflammatidon in re-challenged mice

Am J Transl Res. 2017 Dec 15;9(12):5347-5360. eCollection 2017.

Authors

Rong Zhu¹, Xiaowei Cai¹, Chunli Zhou¹, Yashu Li², Xiaorong Zhang², Yiqian Li¹, Lei Ran¹, Ke Huang¹, Weifeng He², Rupeng Wang¹

Affiliations

¹ Department of Dermatology and Rheumatology Immunology, Xinqiao Hospital, Third Military Medical UniversityChongqing 400037, China.
² Institute of Burn Research, Southwest Hospital, Third Military Medical UniversityChongqing 400038, China.

PMID: 29312488
PMCID: PMC5752886

Abstract

To investigate the role of dermal Vγ₄⁺γδ T cells in psoriasis-like skin inflammation induced by a re-challenge with imiquimod (IMQ), we compared the development of dermatitis induced by topical application of IMQ in primary challenged mice and re-challenged mice. We also compared the development of dermatitis induced by IMQ between re-challenged control mice and Vγ₄^- depleted re-challenged mice that had been initially subjected to IMQ-induced dermatitis 30 prior. We found that the IMQ-induced dermatitis was exacerbated in the re-challenged group compared with the primary challenged group and the Vγ₄^- depleted re-challenged group. In addition, the Vγ₄⁺γδ T cells increased in number and secreted more IL-17A, γ-IFN and IL-22 in the re-challenged control group compared with the primary challenged group. However, in the Vγ₄^- depleted re-challenged group, the Vγ₄^-γδ T cells increased in number and produced more IL-17A and IL-22 compared with re-challenged control mice. These findings suggest that dermal Vγ₄⁺γδ T cells enhance relapsing psoriasis-like skin inflammation induced by IMQ in C57BL/6 mice by secreting IL-17A and γ-IFN.

Keywords: Psoriasis; Vγ4+γδ T cells; cytokines; relapse.