Replanted teeth stored in a newly developed powdered coconut water formula

Dent Traumatol. 2018 Apr;34(2):114-119. doi: 10.1111/edt.12383. Epub 2018 Feb 27.


Background/aim: A newly developed powdered coconut water formulae (ACP-404) has been proposed to preserve periodontal ligament cell viability. The aim of this study was to investigate the periodontal healing of dog teeth replanted after storage in ACP-404 with 2 different osmolalities.

Materials and methods: Sixty teeth of 10 adult beagle dogs were extracted and randomly subjected to the following protocols: immediate replantation after extraction (positive control); dry conditions (negative control); and storage in ACP-404 I (G1), ACP-404 II (G2), in coconut water long shelf life (G3) or in pH-neutralized coconut water long shelf life (G4) for 50 minutes before replantation. The animals were euthanized 28 days post-operatively and processed for histomorphometric analysis using an optical microscope. The groups were subjected to histomorphometric analysis compared with immediate replantation and dry conditions. The data were statistically analysed using the Kruskal-Wallis test with post-analysis using Dunnett's test.

Results: The normal periodontal area was significantly higher for the G1 than for the G2 and G3 groups (P < .001). The positive control group presented similar normal periodontal areas compared to the experimental groups, except for the G3 and negative control groups (P < .001). G1 presented the lowest level of external root replacement resorption compared to the other groups (P < .05). All storage solutions had similar periodontal conditions for repaired resorption areas, inflammatory root resorption, ankylosis and replacement resorption (P > .05).

Conclusions: ACP-404 I (G1) was better overall and was able to preserve the normal periodontium and had less external root replacement resorption.

Keywords: avulsed tooth; histological analysis; replantation; storage media.

MeSH terms

  • Animals
  • Cell Survival
  • Cocos*
  • Dogs
  • Organ Preservation Solutions*
  • Periodontal Ligament / cytology*
  • Random Allocation
  • Tooth Replantation*


  • Organ Preservation Solutions