The risk of infection with viral hepatitis B (HB) in medical personnel of various professions was determined. Differences in the detection of hepatitis B virus markers in medical workers of Moscow and the town of Kzyl-Orda were demonstrated. The maximum number of persons with anti-HBs were found among laboratory personnel (30.8%), physicians (21.5%), hospital attendants (18.8%). The level of detected HBV markers correlated with the degree of contacts with blood. The rate of anti-HBs detection increased with the duration of work in the public health system and reached the maximum levels in the group of persons with a work record of 15-19 years. Comparative evaluation of counter-current immunoelectrophoresis, indirect hemagglutination, and ELISA methods for the detection of HBsAg was made.