RNA-Templated Concatenation of Triplet Nucleic-Acid Probe

Chembiochem. 2018 Apr 4;19(7):674-678. doi: 10.1002/cbic.201700574. Epub 2018 Feb 12.

Abstract

Template-directed synthesis offers several distinct benefits over conventional laboratory creation, including unsurpassed reaction rate and selectivity. Although it is central to many biological processes, such an approach has rarely been applied to the in situ synthesis and recognition of biomedically relevant target. Towards this goal, we report the development of a three-codon nucleic-acid probe containing a C-terminal thioester group and an N-terminal cysteine that is capable of undergoing template-directed oligomerization in the presence of an RNA target and self-deactivation in its absence. The work has implications for the development of millamolecular nucleic-acid probes for targeting RNA-repeated expansions associated with myotonic dystrophy type 1 and other related neuromuscular and neurodegenerative disorders.

Keywords: RNA; concatenation; native chemical ligation; peptide nucleic acids; template synthesis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Codon
  • Cysteine / chemistry
  • Nucleic Acid Hybridization
  • Peptide Nucleic Acids / chemical synthesis
  • Peptide Nucleic Acids / chemistry*
  • Peptide Nucleic Acids / genetics
  • Polymerization
  • RNA / chemistry*
  • RNA / genetics
  • RNA Probes / chemical synthesis
  • RNA Probes / chemistry*
  • RNA Probes / genetics
  • Transition Temperature

Substances

  • Codon
  • Peptide Nucleic Acids
  • RNA Probes
  • RNA
  • Cysteine