Abstract
To improve the diagnosis, surveillance, and control for the rabies virus, a kit for hybridization-triggered fluorescence detection of rabies virus DNA by the RT-PCR technique was developed and evaluated. The analytical sensitivity of the kit was 4*10 GE per ml. High specificity of the kit was shown using representative sampling of viral, bacterial, and human nucleic acids.
MeSH terms
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Animals
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Cats
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DNA Primers / chemical synthesis
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DNA Primers / genetics
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Deer / virology
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Dogs
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Foxes / virology
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Humans
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RNA, Viral / genetics*
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Rabies / diagnosis
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Rabies / epidemiology*
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Rabies / transmission
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Rabies / veterinary*
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Rabies virus / genetics*
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Rabies virus / isolation & purification
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Real-Time Polymerase Chain Reaction / methods
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Real-Time Polymerase Chain Reaction / standards*
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Reverse Transcriptase Polymerase Chain Reaction / standards*
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Russia / epidemiology
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Sensitivity and Specificity