The emission spectra of 70 bioluminescent marine species were measured with a computer controlled optical multichannel analyzer (OMA). A 350 nm spectral window is simultaneously measured using a linear array of 700 silicon photodiodes, coupled by fiber optics to a microchannel plate image intensifier on which a polychromator generated spectrum is focused. Collection optics include a quartz fiber optic bundle which allows spectra to be measured from single photophores. Since corrections are not required for temporal variations in emissions, it was possible to acquire spectra of transient luminescent events that would be difficult or impossible to record with conventional techniques. Use of this system at sea on freshly trawled material and in the laboratory has permitted acquisition of a large collection of bioluminescence spectra of precision rarely obtained previously with such material. Among unusual spectral features revealed were organisms capable of emitting more than one color, including: Umbellula magniflora and Stachyptilum superbum (Pennatulacea), Parazoanthus lucificum (Zoantharia), and Cleidopus gloria-maris (Pisces). Evidence is presented that the narrow bandwidth of the emission spectrum for Argyropelecus affinis (Pisces) is due to filters in the photophores.