PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation

Stem Cell Res. 2018 Mar;27:46-56. doi: 10.1016/j.scr.2017.12.016. Epub 2018 Jan 4.

Abstract

Germ cell tumors (GCTs) are a heterogeneous group of tumors occurring in gonadal and extragonadal locations. GCTs are hypothesized to arise from primordial germ cells (PGCs), which fail to differentiate. One recently identified susceptibility loci for human GCT is PR (PRDI-BF1 and RIZ) domain proteins 14 (PRDM14). PRDM14 is expressed in early primate PGCs and is repressed as PGCs differentiate. To examine PRDM14 in human GCTs we profiled human GCT cell lines and patient samples and discovered that PRDM14 is expressed in embryonal carcinoma cell lines, embryonal carcinomas, seminomas, intracranial germinomas and yolk sac tumors, but is not expressed in teratomas. To model constitutive overexpression in human PGCs, we generated PGC-like cells (PGCLCs) from human pluripotent stem cells (PSCs) and discovered that elevated expression of PRDM14 does not block early PGC formation. Instead, we show that elevated PRDM14 in PGCLCs causes proliferation and differentiation defects in the germline.

Keywords: Cell differentiation; Germ cell tumor; PRDM14; Primordial germ cell; Proliferation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Cell Differentiation / genetics
  • Cell Differentiation / physiology
  • Cell Proliferation / genetics
  • Cell Proliferation / physiology
  • Child
  • Child, Preschool
  • DNA-Binding Proteins
  • Female
  • Germ Cells
  • Humans
  • Infant
  • Male
  • Neoplasms, Germ Cell and Embryonal / genetics
  • Neoplasms, Germ Cell and Embryonal / metabolism*
  • Pluripotent Stem Cells / metabolism
  • RNA-Binding Proteins
  • Repressor Proteins / metabolism*
  • Teratoma / metabolism
  • Transcription Factors
  • Young Adult

Substances

  • DNA-Binding Proteins
  • PRDM14 protein, human
  • RNA-Binding Proteins
  • Repressor Proteins
  • Transcription Factors