D2 dopamine receptor-mediated inhibition of forskolin-stimulated adenylate cyclase activity in rat striatum

Neurosci Lett. 1985 Aug 30;59(2):177-82. doi: 10.1016/0304-3940(85)90196-x.

Abstract

Forskolin markedly stimulates striatal adenylate cyclase activity in a concentration-dependent manner, and at 10(-4) M produces an approximate 40-fold increase in enzyme activity above basal levels. Dopamine (in the presence of 100 nM SCH 23390), bromocryptine and quinpirole (LY 171555) significantly inhibit both basal and forskolin-stimulated adenylate cyclase activity. There is a significant increase in the absolute but not in the percent inhibition of enzyme activity by dopaminergic agonists as a function of forskolin concentration. This inhibition is agonist-concentration dependent and antagonized by the D2 antagonist, spiperone. These results suggest that forskolin may be used as a tool for amplifying the abolute D2-receptor-mediated inhibition of adenylate cyclase in rat striatal homogenates.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenylyl Cyclases / metabolism*
  • Animals
  • Benzazepines / pharmacology
  • Bromocriptine / pharmacology
  • Butyrophenones / pharmacology*
  • Colforsin / antagonists & inhibitors
  • Colforsin / pharmacology*
  • Corpus Striatum / enzymology*
  • Ergolines / pharmacology
  • In Vitro Techniques
  • Male
  • Quinpirole
  • Rats
  • Rats, Inbred Strains
  • Receptors, Dopamine / drug effects*
  • Receptors, Dopamine D2
  • Spiperone / pharmacology*

Substances

  • Benzazepines
  • Butyrophenones
  • Ergolines
  • Receptors, Dopamine
  • Receptors, Dopamine D2
  • Colforsin
  • Quinpirole
  • Bromocriptine
  • Spiperone
  • Adenylyl Cyclases