Meta-analysis of mRNA expression profiles to identify differentially expressed genes in lung adenocarcinoma tissue from smokers and non-smokers

Oncol Rep. 2018 Mar;39(3):929-938. doi: 10.3892/or.2018.6197. Epub 2018 Jan 8.


Compared to other types of lung cancer, lung adenocarcinoma patients with a history of smoking have a poor prognosis during the treatment of lung cancer. How lung adenocarcinoma-related genes are differentially expressed between smoker and non-smoker patients has yet to be fully elucidated. We performed a meta-analysis of four publicly available microarray datasets related to lung adenocarcinoma tissue in patients with a history of smoking using R statistical software. The top 50 differentially expressed genes (DEGs) in smoking vs. non‑smoking patients are shown using heat maps. Additionally, we conducted KEGG and GO analyses. In addition, we performed a PPI network analysis for 8 genes that were selected during a previous analysis. We identified a total of 2,932 DEGs (1,806 upregulated, 1,126 downregulated) and five genes (CDC45, CDC20, ANAPC7, CDC6, ESPL1) that may link lung adenocarcinoma to smoking history. Our study may provide new insights into the complex mechanisms of lung adenocarcinoma in smoking patients, and our novel gene expression signatures will be useful for future clinical studies.

Publication types

  • Meta-Analysis

MeSH terms

  • Adenocarcinoma / chemically induced
  • Adenocarcinoma / genetics*
  • Adenocarcinoma / pathology
  • Biomarkers, Tumor / genetics*
  • Case-Control Studies
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic / drug effects*
  • Humans
  • Lung Neoplasms / chemically induced
  • Lung Neoplasms / genetics*
  • Lung Neoplasms / pathology
  • Prognosis
  • RNA, Messenger / genetics*
  • Smokers / statistics & numerical data*
  • Smoking / adverse effects*
  • Transcriptome


  • Biomarkers, Tumor
  • RNA, Messenger