B cell activation in the cecal patches during the development of an experimental colitis model

Biochem Biophys Res Commun. 2018 Feb 5;496(2):367-373. doi: 10.1016/j.bbrc.2018.01.053. Epub 2018 Jan 10.

Abstract

Although previous studies have suggested that appendix seems to be involved in the colitis, the role of this in the pathogenesis remains unclear. In this study, we assessed the importance of appendiceal lymphoid follicles, specifically the cecal patches (CP) in mice, using an experimental colitis model. Treatment with oxazolone resulted in ulcerations particularly at CP with follicular expansion as well as colitis. The colitis was attenuated by either appendectomy or the absence of mature B cells. We therefore established an intravital imaging system accompanied by the fluorescence resonance energy transfer technology to analyze the dynamic immune response of CP B cells. Our observation revealed frequent Ca2+ signaling in CP B cells during the early phase of colitis development. These findings suggested that the CP B cells may be involved in the pathogenesis of colitis including inflammatory bowel diseases in humans.

Keywords: B cells; Cecal patches; Colitis; Intravital imaging; Oxazolone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Appendix / diagnostic imaging
  • Appendix / immunology*
  • Appendix / pathology
  • B-Lymphocytes / immunology
  • B-Lymphocytes / pathology
  • Calcium Signaling
  • Cecum / diagnostic imaging
  • Cecum / immunology*
  • Cecum / pathology
  • Colitis / chemically induced
  • Colitis / diagnostic imaging
  • Colitis / immunology*
  • Colitis / pathology
  • Colon / diagnostic imaging
  • Colon / immunology*
  • Colon / pathology
  • Disease Models, Animal
  • Humans
  • Intravital Microscopy
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred C57BL
  • Oxazolone
  • Tertiary Lymphoid Structures / diagnostic imaging
  • Tertiary Lymphoid Structures / immunology*
  • Tertiary Lymphoid Structures / pathology

Substances

  • Oxazolone