Evaluation of MALDI-TOF mass spectrometry for the identification of bacteria growing as biofilms

J Microbiol Methods. 2018 Feb:145:79-81. doi: 10.1016/j.mimet.2018.01.003. Epub 2018 Jan 10.

Abstract

We evaluated MALDI-TOF mass spectrometry to identify bacteria from biofilms. We compared three sample preparation procedures on biofilms grown in vitro. The extended direct transfer method was able to identify 13 isolates out of 18 (72%) at the species level and 15 out of 18 (83%) at the genus level.

Keywords: Biofilm; Identification; MALDI-TOF MS.

Publication types

  • Evaluation Study

MeSH terms

  • Acetonitriles / pharmacology
  • Biofilms / drug effects
  • Biofilms / growth & development*
  • Coumaric Acids / pharmacology
  • Ethanol / pharmacology
  • Formates / pharmacology
  • Gram-Negative Bacteria / drug effects
  • Gram-Negative Bacteria / isolation & purification*
  • Gram-Positive Bacteria / drug effects
  • Gram-Positive Bacteria / isolation & purification*
  • Microbiological Techniques / methods*
  • Microbiological Techniques / veterinary
  • Reference Standards
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / veterinary
  • Trifluoroacetic Acid / pharmacology

Substances

  • Acetonitriles
  • Coumaric Acids
  • Formates
  • formic acid
  • alpha-cyano-4-hydroxycinnamate
  • Ethanol
  • Trifluoroacetic Acid
  • acetonitrile