New robust sensitive fluorescence spectroscopy coupled with PLSR for estimation of quercetin in Ziziphus mucronata and Ziziphus sativa

Javid Hussain; Fazal Mabood; Ahmed Al-Harrasi; Liaqat Ali; Tania Shamim Rizvi; Farah Jabeen; Syed Abdullah Gilani; Shehla Shinwari; Mushtaq Ahmad; Zahra Khalfan Alabri; Said Hamood Salim Al Ghawi

doi:10.1016/j.saa.2018.01.002

New robust sensitive fluorescence spectroscopy coupled with PLSR for estimation of quercetin in Ziziphus mucronata and Ziziphus sativa

Spectrochim Acta A Mol Biomol Spectrosc. 2018 Apr 5:194:152-157. doi: 10.1016/j.saa.2018.01.002. Epub 2018 Jan 6.

Authors

Affiliations

¹ Department of Biological Sciences & Chemistry, College of Arts and Sciences, University of Nizwa, Oman. Electronic address: javidhej@unizwa.edu.om.
² Department of Biological Sciences & Chemistry, College of Arts and Sciences, University of Nizwa, Oman. Electronic address: mehboob@unizwa.edu.om.
³ UoN Chair of Oman's Medicinal Plants and Marine Natural Products, University of Nizwa, Oman. Electronic address: aharrasi@unizwa.edu.om.
⁴ UoN Chair of Oman's Medicinal Plants and Marine Natural Products, University of Nizwa, Oman; Department of Chemistry, University of Sargodha, Sub-campus Mianwali, Pakistan.
⁵ UoN Chair of Oman's Medicinal Plants and Marine Natural Products, University of Nizwa, Oman.
⁶ Department of Chemistry, University of Malakand, KPK, Pakistan.
⁷ Department of Biological Sciences & Chemistry, College of Arts and Sciences, University of Nizwa, Oman.
⁸ Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.

PMID: 29331816
DOI: 10.1016/j.saa.2018.01.002

Abstract

Flavonoids are natural antioxidants derived from plants and commonly found in a variety of foods to sequester free radicals. Quercetin, belonging to flavonol subclass of flavonoids, has received considerable attention because of its wide uses as a nutritional supplement as well as a phytochemical remedy for a number of diseases. In the current study, quantification of quercetin was carried out in two medicinally important flavonoid rich plant Ziziphus mucronata and Ziziphus sativa. Emission spectroscopy was utilized as a new method coupled with Partial Least Squares Regression (PLSR) and the cross validation was done by UV-Visible spectroscopy. The results indicated the higher quercetin content in Z. mucronata (1.50±0.034%) than Z. sativa (1.21±0.052%), and were further verified through Folin-Ciocalteu Colorimetric method (Z. mucronata; 1.41±0.26% and Z. sativa; 1.13±0.136%). In this study the sensitivity was explained in term of slope i.e. Slope=0.9973.

Keywords: Fluorescence spectroscopy; PLS regression; Quercetin; UV–VIS spectroscopy; Ziziphus mucronata; Ziziphus sativa.

MeSH terms

Antioxidants / analysis*
Least-Squares Analysis*
Quercetin / analysis*
Spectrometry, Fluorescence / methods*
Ziziphus / classification
Ziziphus / metabolism*

Substances

Antioxidants
Quercetin