Effect of Bioactive Compound of Aronia melanocarpa on Cardiovascular System in Experimental Hypertension

Oxid Med Cell Longev. 2017;2017:8156594. doi: 10.1155/2017/8156594. Epub 2017 Nov 30.

Abstract

Aronia melanocarpa has attracted scientific interest due to its dense contents of different polyphenols. We aimed to analyse effects of Aronia melanocarpa (AME) extract on blood pressure (BP), lipid peroxidation, cytokine level, total NOS activity in the left ventricle (LV), and aorta of L-NAME-induced hypertensive rats. 12-week-old male WKY rats were assigned to the control group and groups treated with AME extract (57.90 mg/kg/day), L-NAME (40 mg/kg/day), or combination of L-NAME (40 mg/kg/day) and AME (57.90 mg/kg/day) in tap water for 3 weeks. NOS activity, eNOS protein expression, and conjugated diene (CD) concentration were determined in the LV and aorta. After 3 weeks of L-NAME treatment, BP was increased by 28% and concomitant treatment with AME reduced it by 21%. NOS activity of the LV and aorta in the L-NAME group was decreased by about 40%, while AME increased it almost on the control level. AME-induced eNOS upregulation may contribute to increase NOS activity. Moreover, AME decreased CD concentration in the LV and aorta and TNF-α and IL-6 production in the plasma were increased by L-NAME treatment. In conclusion, our results showed that active substances of Aronia melanocarpa may have a positive effect on blood pressure, NOS activity, and proinflammatory processes in L-NAME-induced hypertension.

MeSH terms

  • Alkadienes / analysis
  • Alkadienes / metabolism
  • Animals
  • Aorta / chemistry
  • Aorta / drug effects*
  • Aorta / enzymology
  • Blood Pressure / drug effects
  • Heart Ventricles / chemistry
  • Heart Ventricles / drug effects*
  • Heart Ventricles / enzymology
  • Hypertension / etiology
  • Hypertension / prevention & control
  • Hypertension / veterinary
  • Interleukin-6 / blood
  • Male
  • NG-Nitroarginine Methyl Ester / toxicity
  • Nitric Oxide Synthase Type II / genetics
  • Nitric Oxide Synthase Type II / metabolism
  • Nitric Oxide Synthase Type III / genetics
  • Nitric Oxide Synthase Type III / metabolism
  • Photinia / chemistry*
  • Photinia / metabolism
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology*
  • Plant Extracts / therapeutic use
  • Polyphenols / chemistry
  • Polyphenols / pharmacology
  • Polyphenols / therapeutic use
  • Rats
  • Rats, Inbred WKY
  • Tumor Necrosis Factor-alpha / metabolism
  • Up-Regulation / drug effects*

Substances

  • Alkadienes
  • Interleukin-6
  • Plant Extracts
  • Polyphenols
  • Tumor Necrosis Factor-alpha
  • Nitric Oxide Synthase Type II
  • Nitric Oxide Synthase Type III
  • NG-Nitroarginine Methyl Ester