Oral Microbiome Characterization in Murine Models

Bio Protoc. 2017 Dec 20;7(24):e2655. doi: 10.21769/BioProtoc.2655.


The oral microbiome has been implicated as a trigger for immune responsiveness in the oral cavity, particularly in the setting of the inflammatory disease periodontitis. The protocol presented here is aimed at characterizing the oral microbiome in murine models at steady state and during perturbations of immunity or physiology. Herein, we describe murine oral microbiome sampling procedures, processing of low biomass samples and subsequent microbiome characterization based on 16S rRNA gene sequencing.

Keywords: 16S rRNA gene sequencing; Gingival microbiome; Microbiome sequencing; Mouse oral sampling; Murine oral microbiota; Oral microbiome.