Complete Genome Sequencing of the Luminescent Bacterium, Vibrio Qinghaiensis Sp. Q67 Using PacBio Technology

Sci Data. 2018 Jan 16;5:170205. doi: 10.1038/sdata.2017.205.

Abstract

Vibrio qinghaiensis sp.-Q67 (Vqin-Q67) is a freshwater luminescent bacterium that continuously emits blue-green light (485 nm). The bacterium has been widely used for detecting toxic contaminants. Here, we report the complete genome sequence of Vqin-Q67, obtained using third-generation PacBio sequencing technology. Continuous long reads were attained from three PacBio sequencing runs and reads >500 bp with a quality value of >0.75 were merged together into a single dataset. This resultant highly-contiguous de novo assembly has no genome gaps, and comprises two chromosomes with substantial genetic information, including protein-coding genes, non-coding RNA, transposon and gene islands. Our dataset can be useful as a comparative genome for evolution and speciation studies, as well as for the analysis of protein-coding gene families, the pathogenicity of different Vibrio species in fish, the evolution of non-coding RNA and transposon, and the regulation of gene expression in relation to the bioluminescence of Vqin-Q67.

Publication types

  • Dataset
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Genome, Bacterial*
  • Vibrio / classification
  • Vibrio / genetics*
  • Whole Genome Sequencing / methods