A new genetically engineered mouse model of choroid plexus carcinoma

Biochem Biophys Res Commun. 2018 Feb 5;496(2):568-574. doi: 10.1016/j.bbrc.2017.11.192. Epub 2018 Jan 12.

Abstract

Choroid plexus carcinomas (CPCs) are highly malignant brain tumours predominantly found in children and associated to poor prognosis. Improved therapy for these cancers would benefit from the generation of animal models. Here we have created a novel mouse CPC model by expressing a stabilised form of c-Myc (MycT58A) and inactivating Trp53 in the choroid plexus of newborn mice. This induced aberrant proliferation of choroid plexus epithelial cells, leading to aggressive tumour development and death within 150 days. Choroid plexus tumours occurred with a complete penetrance in all brain ventricles, with prevalence in the lateral and fourth ventricles. Histological and cellular analysis indicated that these tumours were CPCs resembling their human counterparts. Comparison of gene expression profiles of CPCs and non-neoplastic tissues revealed profound alterations in cell cycle regulation and DNA damage responses, suggesting that dysregulation of cell division and DNA checkpoint pathways may represent key vulnerabilities. This novel animal model of CPC provides an invaluable tool to elucidate the mechanism of CPC formation and to develop successful therapies against this devastating paediatric cancer.

Keywords: Choroid plexus carcinoma; Mouse model; Myc; Otx2; Paediatric cancer; Trp53.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carcinogenesis / genetics
  • Carcinogenesis / pathology
  • Carcinoma / genetics*
  • Carcinoma / pathology*
  • Cell Proliferation
  • Choroid Plexus / pathology*
  • Choroid Plexus Neoplasms / genetics*
  • Choroid Plexus Neoplasms / pathology*
  • DNA Damage
  • Disease Models, Animal
  • Humans
  • Mice
  • Mice, Transgenic
  • Mutation
  • Proto-Oncogene Proteins c-myc / genetics*
  • Transcriptome
  • Tumor Suppressor Protein p53 / genetics*

Substances

  • Proto-Oncogene Proteins c-myc
  • Tumor Suppressor Protein p53

Supplementary concepts

  • Choroid Plexus Carcinoma