Pterostilbene, a Potential MCR-1 Inhibitor That Enhances the Efficacy of Polymyxin B

Antimicrob Agents Chemother. 2018 Mar 27;62(4):e02146-17. doi: 10.1128/AAC.02146-17. Print 2018 Apr.

Abstract

We characterized the synergistic effect produced between pterostilbene and polymyxin B (fractional inhibitory concentration [FIC] index = 0.156 or 0.188) against MCR-producing Escherichia coli strains of both human and animal origins. The time-killing assays showed that either pterostilbene or polymyxin B failed to eradicate the mcr-1- and NDM-positive E. coli strain ZJ487, but the combination eliminated the strain by 1 h postinoculation. The survival rate of mice after intraperitoneal infections was significantly enhanced from 0% to 60% in the group in which combination therapy was applied.

Keywords: Enterobacteriaceae; MCR-1; inhibitor; polymyxin B; pterostilbene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Enterobacteriaceae / drug effects
  • Enterobacteriaceae / genetics
  • Enterobacteriaceae / pathogenicity
  • Enterobacteriaceae Infections / drug therapy
  • Escherichia coli Proteins / antagonists & inhibitors
  • Female
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Microbial Sensitivity Tests
  • Polymyxin B / therapeutic use*
  • Stilbenes / therapeutic use*

Substances

  • Escherichia coli Proteins
  • MCR-1 protein, E coli
  • Stilbenes
  • pterostilbene
  • Polymyxin B