Osteogenic efficacy of BMP-2 mixed with hydrogel and bone substitute in peri-implant dehiscence defects in dogs: 16 weeks of healing

Clin Oral Implants Res. 2018 Mar;29(3):300-308. doi: 10.1111/clr.13117. Epub 2018 Jan 17.

Abstract

Objectives: The objective of this study was to determine the effect of bone morphogenetic protein-2 (BMP-2) mixed with either polyethylene glycol hydrogel or synthetic bone substitute (SBS) on new bone formation in peri-implant dehiscence defects after 16 weeks of healing.

Materials and methods: A guided bone regeneration procedure was performed in box-type peri-implant defects that were surgically prepared in six beagle dogs. The following four experimental groups were used (i) control (no graft), (ii) SBS+hydrogel, (iii) SBS+BMP-2/hydrogel and (iv) BMP-2/SBS+hydrogel. Volumetric analysis using micro-computed tomography and histomorphometric analysis was performed at 16 weeks post-operatively.

Results: The amount of new bone and the total augmented volume did not differ significantly between both BMP-treated groups and the SBS+hydrogel group (p > .05). Likewise, no histometric differences were observed in the values of new bone area and bone-to-implant contact ratio among the three augmentation groups (new bone area: 0.06 ± 0.08, 0.19 ± 0.20, 0.48 ± 0.37 and 0.56 ± 0.60 mm2 [mean ± standard deviation] in groups 1-4, respectively; bone-to-implant contact: 9.44 ± 11.51%, 19.91 ± 15.19%, 46.31 ± 29.82% and 42.58 ± 26.27% in groups 1-4, respectively).

Conclusion: The osteogenic efficacy of BMP-2 on the regeneration of peri-implant bone defects was not detectable after 16 weeks regardless of the carrier materials.

Keywords: bone morphogenetic protein-2; bone substitutes; dental implants; hydrogel; polyethylene glycol.

MeSH terms

  • Alveolar Ridge Augmentation
  • Animals
  • Bone Morphogenetic Protein 2 / pharmacology*
  • Bone Regeneration / drug effects*
  • Bone Substitutes / pharmacology*
  • Bone Transplantation
  • Cone-Beam Computed Tomography / methods
  • Dental Implantation, Endosseous / methods*
  • Dental Implants*
  • Dogs
  • Drug Combinations
  • Guided Tissue Regeneration / methods
  • Hydrogel, Polyethylene Glycol Dimethacrylate / pharmacology*
  • Hydrogels
  • Imaging, Three-Dimensional
  • Male
  • Mandible / diagnostic imaging
  • Mandible / pathology
  • Mandible / surgery
  • Models, Animal
  • Osteogenesis / drug effects*
  • Surgical Wound Dehiscence / diagnostic imaging
  • Surgical Wound Dehiscence / drug therapy*
  • Wound Healing / drug effects
  • X-Ray Microtomography

Substances

  • Bone Morphogenetic Protein 2
  • Bone Substitutes
  • Dental Implants
  • Drug Combinations
  • Hydrogels
  • Hydrogel, Polyethylene Glycol Dimethacrylate