Impaired intracellular transport of class I MHC antigens as a possible means for adenoviruses to evade immune surveillance

Cell. 1985 Nov;43(1):215-22. doi: 10.1016/0092-8674(85)90026-1.

Abstract

Early region E3 of adenovirus-2 encodes a glycoprotein, E19, that associates with class I MHC antigens. The interaction occurs concomitantly with abrogated terminal glycosylation of class I antigen heavy chains, as shown by pulse-chase and Endo H digestion experiments. Also the viral protein remains in the high mannose form throughout the chase period. These results do not reflect a general impairment of terminal glycosylation of membrane proteins. Immunofluorescence analyses demonstrated that in virally infected cells E19 and class I antigens accumulate in the perinuclear region. Class I antigens gradually disappear from the cell surface. A diminished cell surface expression of class I antigens may be a means by which adenoviruses evade immune surveillance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenovirus Early Proteins
  • Adenoviruses, Human / immunology*
  • Adenoviruses, Human / physiology
  • Biological Transport
  • Cell Membrane / immunology*
  • HLA Antigens*
  • HeLa Cells
  • Hexosaminidases / metabolism
  • Humans
  • Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
  • Membrane Proteins / metabolism
  • Oncogene Proteins, Viral / metabolism*
  • Transfection
  • Viral Proteins / metabolism*

Substances

  • Adenovirus Early Proteins
  • HLA Antigens
  • Membrane Proteins
  • Oncogene Proteins, Viral
  • Viral Proteins
  • Hexosaminidases
  • Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase